常年发情和季节性发情绵羊在不同季节生殖激素变化规律

doi:10.11843/j.issn.0366-6964.2013.10.006

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (10): 1547-1553.doi: 10.11843/j.issn.0366-6964.2013.10.006

常年发情和季节性发情绵羊在不同季节生殖激素变化规律

贺建宁¹，王金鑫¹，狄冉^1*，王耀武¹，孙晓笛²，杨志敏²，刘秋月¹，张鑫荣³，任德新³，崔保国³，蔡占荣³，储明星^1*

（1. 中国农业科学院北京畜牧兽医研究所，农业部畜禽遗传资源与种质创新重点实验室，北京 100193；2. 扬州大学动物科学与技术学院，扬州 225009； 3. 宁夏盐池滩羊选育场，盐池751506）

收稿日期:2013-05-06 出版日期:2013-10-23 发布日期:2013-10-23
通讯作者: 狄冉，副研究员，E-mail：dirangirl@163.com；储明星，研究员，博士生导师，E-mail：mxchu@263.net
作者简介:贺建宁（1984-），男，陕西靖边人，博士生，主要从事动物遗传育种与繁殖研究，Tel：010-62816001，E-mail：hexingxing104@163.com
基金资助:
国家自然科学基金项目(31101687)；国家现代肉羊产业技术体系建设专项资金(CARS-39)；中央级公益性科研院所基本科研业务费专项(2011cj-7；2013ywf-zd-1)

Seasonal Variations of Reproductive Hormone Level in Year-round andSeasonal Estrus Sheep

HE Jian-ning¹，WANG Jin-xin¹，DI Ran^1*，WANG Yao-wu¹，SUN Xiao-di²，YANG Zhi-min²，LIU Qiu-yue¹，ZHANG Xin-rong³，REN De-xin³，CUI Bao-guo³，CAI Zhan-rong³， CHU Ming-xing^1*

(1. Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China； 3. Breeding Farm of Tan Sheep in Ningxia,Yanchi 751506, China)

Received:2013-05-06 Online:2013-10-23 Published:2013-10-23

摘要/Abstract

摘要：

旨在通过检测一年四季常年发情和季节性发情绵羊外周血液中4种重要生殖激素（雌二醇，E₂)、孕酮(P₄)、促卵泡激素(FSH)和促黄体激素(LH)）的浓度，解析绵羊常年发情的生殖激素变化规律，为调控绵羊的繁殖季节性提供激素方面的理论依据。3～4周岁的小尾寒羊成年母羊和滩羊成年母羊各5只，春、夏、秋和冬4个季节颈静脉采血，采用放射免疫法测定E₂、P₄、FSH和LH的浓度。试情公羊爬跨法表明小尾寒羊4个季节均可发情，而滩羊从8月份开始发情至来年3月份陆续进入乏情期。小尾寒羊外周血液中4种生殖激素在春、夏、秋、冬4个季节变化基本稳定。滩羊生殖激素变化具有显著的季节性，E₂变化基本稳定；P₄分泌量秋冬季节显著高于春夏季节（P<0.05）；FSH和LH分泌量秋冬季节显著高于春季和夏季（P<0.05），夏季显著高于春季（P<0.05）。通过对2品种间激素变化研究发现，小尾寒羊与滩羊4个季节血液中E₂浓度差异不显著（P>0.05）；春夏季节时小尾寒羊 P₄和FSH含量显著高于滩羊（P<0.05）；小尾寒羊 LH含量在4个季节均高于滩羊，但仅在冬季时差异显著（P<0.05）。上述结果表明，稳定的E₂水平及一定浓度的P₄可能与绵羊的常年发情有关，高水平的FSH和LH对开启或维持绵羊的发情具有重要作用,或与多胎具有一定的关系。

Abstract:

The objective of this study is to analyze the reproductive hormone seasonal variations of year-round and seasonal estrous sheep. E₂, P₄, FSH and LH concentration in serum were determined, and the results could provide theoretical basis of hormone for regulating the seasonal reproduction in sheep. Blood samples were collected from five adult Small-tailed Han ewes and five adult Tan ewes in spring, summer, autumn and winter, respectively. The serum E₂, P₄, FSH and LH were measured by radioimmunoassay. According to the observation, the Small-tailed Han sheep kept estrous status during four seasons, while the Tan sheep started estrus in August and ended in March. The results showed that these four hormone level in Small-tailed Han sheep had no significant changes around the year. However, it was different in Tan sheep, P₄, FSH and LH concentration in estrous season were significantly higher(P<0.05) than those in anestrous season. According to the analysis between two breeds, E₂ level has no significant difference（P>0.05）around the year. The serum P₄and FSH concentration in Small-tailed Han sheep were significantly higher(P<0.05) than in Tan sheep during spring and summer. The LH level in Small-tailed Han sheep was higher than in Tan sheep, however, which has no significant difference (P>0.05) between two breeds except in winter. These results indicated that a certain level of E₂ and P₄ were needed for sheep year-round estrous, and high level of FSH and LH were necessary for year-round estrus or polyembryony in sheep.

中图分类号:

S826
S814.1

贺建宁，王金鑫，狄冉，王耀武，孙晓笛，杨志敏，刘秋月，张鑫荣，任德新，崔保国，蔡占荣，储明星. 常年发情和季节性发情绵羊在不同季节生殖激素变化规律[J]. 畜牧兽医学报, 2013, 44(10): 1547-1553.

HE Jian-ning，WANG Jin-xin，DI Ran，WANG Yao-wu，SUN Xiao-di，YANG Zhi-min，LIU Qiu-yue，ZHANG Xin-rong，REN De-xin，CUI Bao-guo，CAI Zhan-rong， CHU Ming-xing. Seasonal Variations of Reproductive Hormone Level in Year-round andSeasonal Estrus Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1547-1553.

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常年发情和季节性发情绵羊在不同季节生殖激素变化规律

Seasonal Variations of Reproductive Hormone Level in Year-round andSeasonal Estrus Sheep

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